Plant Tissue
Culture

 Plant Molecular
Biology

Seed   Maintenance and  storage

 


 
 

 



 

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  • DNA extraction, using a protocol modified from Doyle et al  1991

  • Restriction endonuclease digestion of the DNA and ligation of adapters

  • Amplification of the restriction fragments

  • Gel analysis of the amplified fragment, using a polyacrylamide gel. Typically, 50 to 100 restriction fragments are complified in each AFLP reaction.

  • artocapus heterophyllus  Lamk. (Jackfruit)

  • Calamus  sp. (Rattan)

  • Aegle marmelos  (L.) Corr. (Bel Fruit)

  • Olea europaea  L. (Olive)

  • Dorotis  sp.


 

     
Plant material (leaves) Grind the plant material in a mortar Transfer the ground tissue to eppendorf tube, add 1 ml. extraction buffer and precipitate DNA
 
   
 
  Visualise the DNA on the gel Check DNA concentration on an agarose gel by electrophoresis;
     
 

  Plant Genetic Conservation Project Office
The Royal Chitralada Palace, Dusit , Bangkok 10303 ,Thailand
Tel /Fax : 02-2821850, 02-2820665
E-mail : admin@plantgenetics-rspg.org
 
 
 
 

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