Identification of Plants in Plant
Genetic Conservation Project Using a DNA Fingerprinting Technique (AFLP)
are important constituents of all living cells. There are two types of
nucleic acid in living organisms: deoxyribonucleic acid (DNA) and
ribonucleic acid (RNA) . Genes are stored within organism as DNA and when
the gene is active it will be translated into RNA and finally protein.
be used to determine genetic markers with molecular marker technologies.
avoid many of the complication of environmental effects acting upon
characters by looking directly at variation controlled by genes or DNA.
developments have expanded the range of DNA polymorphism assays for genetic
mapping. This has allowed mapping to assist with plant breeding,
genome fingerprinting and for investigating genetic relatedness.
RFLP ( Restriction
Fragment Length Ploymorphism )
RAPD ( Random-amplified
Polymorphic DNA )
AFLP ( Amplified Fragment
Length Ploymorphism )
SSR ( Simple Seguence Repeat,
To describe the applications of
molecular marker technologies for plant identification
To reveal the differences between
plants at the genetic level
To use mapping for plant improvement
- Detect somaclonal variation in the case of in vitro
- As certain the linkage between DNA markers and special
characters of plant species, such as disease resistance and insect
- To help in the classical methods of estimating the genetic
diversity, using a powerful and rapid method to screen and select
germplasm within breeding programmes
- To collect DNA fingerprint data for legal and patent protection